H2N2V1, Main, Exploration, bibRecord, 002387

Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions

Identifieur interne : 002387 ( Main/Exploration ); précédent : 002386; suivant : 002388

Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions

Auteurs : P. Pothier [France] ; G. A. Denoyel [France] ; S. Ghim [France] ; G. Prudhomme De Saint Maur [France] ; F. Freymuth [France]

Source :

European Journal of Clinical Microbiology [ 0722-2211 ] ; 1986-06-01.

RBID : ISTEX:ED3F373AC320FC327D8E04170F1622530F4D3EA8

English descriptors

Teeft :
- Antibody, Antibody titer, Antigenic, Antigenic variation, Ascitic fluid, Cases supernatants, Clinical microbiology, Clinical specimens, Culture medium, Diagnostics pasteur, Epidemic period, Human subtypes, Immunofluorescence, Immunofluorescence assay, Influenza, Influenza virus, Institut pasteur, Internal antigen, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoelonal antibodies, Multicentre study, Nasal smears, Nasopharyngeal, Nasopharyngeal samples, Nasopharyngeal secretions, Nosocomial influenza infections, Polyclonal, Polyclonal antibodies, Rapid detection, Rapid diagnosis, Respiratory agents, Respiratory syncytial virus, Such antibodies, Supernatant, Surface epitope, Surface proteins, Viral, Viral culture, Viral isolation, Virus infections, Virus nucleoprotein.

Abstract

Abstract: Two monoclonal antibodies against influenza A virus were assessed for use as diagnostic reagents in an indirect immunofluorescence assay (IFA) of nasopharyngeal secretions. Monoclonal antibody IA-52, directed at an internal antigen, reacted with all influenza A tested. The high stability of this epitope permitted its use in a rapid IFA test, which gave results comparable to those obtained with polyclonal antibodies and viral isolation. The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H3 subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1,H3N2).

Url:

https://api.istex.fr/ark:/67375/1BB-60133THC-M/fulltext.pdf

DOI: 10.1007/BF02017792

Affiliations:

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to stream Main, to step Curation: 002387

Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Two monoclonal antibodies against influenza A virus were assessed for use as diagnostic reagents in an indirect immunofluorescence assay (IFA) of nasopharyngeal secretions. Monoclonal antibody IA-52, directed at an internal antigen, reacted with all influenza A tested. The high stability of this epitope permitted its use in a rapid IFA test, which gave results comparable to those obtained with polyclonal antibodies and viral isolation. The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H3 subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1,H3N2).</div>
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Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions

Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri